Procedure for injecting chloroform to a lab animal ?

1. Prepare the chloroform solution by diluting it to the appropriate concentration for the size and species of the lab animal being used.

2. Restrain the lab animal in a suitable restraint device to prevent movement during the injection.

3. Clean the injection site with alcohol or another disinfectant to reduce the risk of infection.

4. Fill a syringe with the prepared chloroform solution, ensuring there are no air bubbles in the syringe.

5. Insert the needle of the syringe into a suitable injection site, such as the intraperitoneal cavity or a vein, depending on the specific requirements of the experiment.

6. Slowly inject the chloroform solution into the lab animal, taking care to avoid causing any pain or distress.

7. Monitor the lab animal closely for signs of anesthesia, such as loss of consciousness or reduced reflexes.

8. Once the desired level of anesthesia is achieved, proceed with the experiment or procedure as planned.

9. Dispose of any unused chloroform solution and properly dispose of the syringe and needle in a sharps container.

10. Monitor the lab animal closely during recovery from anesthesia and provide appropriate care and support as needed.